![]() ![]() The impact of endpoint dilution plate design choices (dilution factor, replicates per dilution) on measurement accuracy is also explored. midSIN’s estimates are shown to be more accurate and robust than the Reed-Muench and Spearman-Kärber approximations. ![]() It can therefore be used directly to achieve a desired multiplicity of infection, similarly to how plaque or focus forming units (PFU, FFU) are used. We analyzed influenza and respiratory syncytial virus samples using midSIN and demonstrated that the SIN/mL reliably corresponds to the number of infections a sample will cause per mL. midSIN computes a virus sample’s SIN concentration using Bayesian inference based on the results of a standard endpoint dilution assay, and requires no changes to current experimental protocols. We introduce a replacement for the ID 50 that we call Specific INfection (SIN) along with a free and open-source web-application, midSIN ( ) to calculate it. The endpoint dilution assay’s output, the 50% infectious dose (ID 50), is calculated using the Reed-Muench or Spearman-Kärber mathematical approximations, which are biased and often miscalculated. ![]()
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